Access Restriction

Author Sun, Lihua ♦ Mi, Chunyi ♦ Hu, Xiufang ♦ Li, Ou ♦ Pei, Cong ♦ Liu, Wenhong
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Taylor & Francis Group
File Format PDF
Date Created 2017-03-03
Copyright Year ©2017
Language English
Subject Domain (in LCC) TP248.13-248.65
Subject Keyword Biotechnology ♦ Diversity ♦ Metagenome ♦ Dendrobium officinale ♦ Chemical technology ♦ Technology ♦ DGGE ♦ Culture-dependent
Abstract Culture-dependent and culture-independent methods were compared and evaluated in the study of the endophytic diversity of Dendrobium officinale. Culture-independent methods consisted of polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) and metagenome methods. According to the results, differences were found between the three methods. Three phyla, namely Firmicutes, Proteobacteria, and Actinobacteria, were detected using the culture-dependent method, and two phyla, Firmicutes and Proteobacteria, were detected by the DGGE method. Using the metagenome method, four major phyla were determined, including Proteobacteria (76.54%), Actinobacteria (18.56%), Firmicutes (2.27%), and Bacteroidetes (1.56%). A distinct trend was obtained at the genus level in terms of the method and the corresponding number of genera determined. There were 449 genera and 16 genera obtained from the metagenome and DGGE methods, respectively, and only 7 genera were obtained through the culture-dependent method. By comparison, all the genera from the culture-dependent and DGGE methods were contained in the members determined using the metagenome method. Overall, culture-dependent methods are limited to ‘finding’ endophytic bacteria in plants. DGGE is an alternative to investigating primary diversity patterns; however, the metagenome method is still the best choice for determining the endophytic profile in plants. It is essential to use multiphasic approaches to study cultured and uncultured microbes.
ISSN 13143530
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2017-01-01
e-ISSN 13102818
Journal Biotechnology & Biotechnological Equipment
Volume Number 31
Issue Number 1
Page Count 8
Starting Page 112
Ending Page 119

Source: Directory of Open Access Journals (DOAJ)