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Author Crathorn, A. R. ♦ Shooter, K. V.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE ♦ ANIMAL CELLS ♦ DISTRIBUTION ♦ LABELLED COMPOUNDS ♦ MEASURED VALUES ♦ METABOLISM ♦ MICE ♦ NUCLEIC ACIDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ QUANTITATIVE ANALYSIS ♦ RADIOACTIVITY ♦ RADIOAUTOGRAPHY ♦ THYMIDINE ♦ TRITIUM
Abstract The uptake of tritium-labelled thymidine by mouse Ehrlich and Landschutz ascites cells in vitro was studied by measuring the activity present in the cell and incorporated into desoxyribonucleic acid (DNA) as a function of time. The distribution of the precursor among the cells was determined by autoradiography. The results show that thymidine is rapidly taken up by cells, but the incorporation rate in DNA is much slower. The precursor studies indicate.that the tritium activity is 15% thymidine di- and tri-phosphates, 25% thymidine monophosphate, and 60% thymidine. The assumption that the activity left in the nucleus after washing and fixing is due to thymidine incorporated into DNA is shown to be invalid; precursors are left behind as well as DNA, and the average activity per cell is about the same for both the two incubation periods and has no relationship to the DNA activity. (D.L.C.)
Educational Use Research
Learning Resource Type Article
Publisher Date 1960-08-13
Publisher Department Royal Cancer Hospital, London
Journal Nature
Volume Number 187
Organization Royal Cancer Hospital, London


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