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Author Cerione, R. A. ♦ Gierschik, P. ♦ Staniszewski, C. ♦ Benovic, J. L. ♦ Codina, J. ♦ Somers, R. ♦ Spiegel, A. M. ♦ Lefkowitz, R. J. ♦ Caron, M. G.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ CYCLASES ♦ BIOCHEMISTRY ♦ INHIBITION ♦ ENZYME ACTIVITY ♦ RADIOASSAY ♦ ATP ♦ BRAIN ♦ CATTLE ♦ DOSE-RESPONSE RELATIONSHIPS ♦ ERYTHROCYTES ♦ GUANINE ♦ INDOLES ♦ IODINE 125 ♦ NUCLEOTIDES ♦ PHOSPHOLIPIDS ♦ PHOSPHORUS 32 ♦ PROTEINS ♦ SULFUR 35 ♦ TRITIUM COMPOUNDS ♦ AMINES ♦ ANIMALS ♦ AROMATICS ♦ AZAARENES ♦ AZOLES ♦ BETA DECAY RADIOISOTOPES ♦ BETA-MINUS DECAY RADIOISOTOPES ♦ BIOLOGICAL MATERIALS ♦ BLOOD ♦ BLOOD CELLS ♦ BODY ♦ BODY FLUIDS ♦ CENTRAL NERVOUS SYSTEM ♦ CHEMISTRY ♦ DAYS LIVING RADIOISOTOPES ♦ DOMESTIC ANIMALS ♦ ELECTRON CAPTURE RADIOISOTOPES ♦ ENZYMES ♦ ESTERS ♦ EVEN-ODD NUCLEI ♦ HETEROCYCLIC COMPOUNDS ♦ HYDROXY COMPOUNDS ♦ INTERMEDIATE MASS NUCLEI ♦ IODINE ISOTOPES ♦ ISOTOPES ♦ LABELLED COMPOUNDS ♦ LIGHT NUCLEI ♦ LIPIDS ♦ LYASES ♦ MAMMALS ♦ MATERIALS ♦ NERVOUS SYSTEM ♦ NUCLEI ♦ ODD-EVEN NUCLEI ♦ ODD-ODD NUCLEI ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ ORGANIC PHOSPHORUS COMPOUNDS ♦ ORGANS ♦ PHOSPHORUS ISOTOPES ♦ PURINES ♦ PYRROLES ♦ RADIOISOTOPES ♦ RUMINANTS ♦ SULFUR ISOTOPES ♦ VERTEBRATES ♦ Biochemistry- Tracer Techniques
Abstract The authors have examined the mechanism of inhibition of adenylate cyclase using the purified ..cap alpha.. and ..beta gamma.. subunits of bovine brain inhibitory guanine nucleotide regulatory protein (N/sub i/) (i.e., ..cap alpha../sub i/ and ..beta gamma../sub N/) and bovine retinal transducin (..cap alpha../sub T/ and ..beta gamma../sub T/) in reconstituted phospholipid vesicle systems. The addition of ..beta gamma../sub N/ or ..beta gamma../sub T/ to lipid vesicles containing the pure stimulatory guanine nucleotide regulatory protein (N/sub s/) from human erythrocytes as well as a resolved preparation of the catalytic moiety (C) of bovine caudate adenylate cyclase results in significant inhibition of guanine nucleotide stimulated cyclase activity (80-90%). The inhibition by these ..beta gamma.. subunit complexes appears to fully account for the inhibitory effects observed with holo-N/sub i/ or holotransducin. A variety of structure-function comparison of the ..beta gamma../sub N/ and ..beta gamma../sub T/ complexes were performed in order to further probe the molecular mechanisms involved in the inhibitory pathway. Whereas the ..beta.. subunits of ..beta gamma../sub N/ and ..beta gamma../sub T/ appear to be very similar, if not identical, on the basis of comparisons of their gel electrophoretic mobility and immunological cross-reactivity, clear differences exist in the apparent structures of ..gamma../sub N/ and ..gamma../sub T/. The functional differences between the ..beta gamma.. subunits of brain N/sub i/ and retinal transducin may reflect the clear structural differences in their ..gamma.. subunits, thus suggesting a possible role for ..gamma.. in the inhibition of adenylate cyclase by N/sub i/. Overall, these results indicate that the ..beta gamma.. complexes, as well as the ..cap alpha.. subunits, may have an important role in imparting the functional specificity necessary for nucleotide regulatory protein mediated signal transduction.
Educational Use Research
Learning Resource Type Article
Publisher Date 1987-03-10
Publisher Place United States
Journal Biochemistry
Volume Number 26
Issue Number 5
Organization Duke Univ. Medical Center, Durham, NC


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