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Author Shindo, H. ♦ Kurumizaka, H. ♦ Furubayashi, A. ♦ Sakuma, C. ♦ Matumoto, U. ♦ Yanagida, A. ♦ Goshima, N. ♦ Kano, Y. ♦ Imamoto, F.
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword NMR ♦ HU protein ♦ protein complex with DNA
Abstract The solution structures of HUα and its complex with oligo DNA were studied by proton NMR spectroscopy. It was confirmed that the arm region of HU forms antiparallel β-sheet and that all of the residues of phenylalanines together with some of leucines and/or valines form a hydrophobic core within the dimer of HU protein. Upon complex formation of HUα with DNA, several resonances from both HU and DNA were perturbed and the intermolecuar NOEs between HU and DNA were observed, suggesting that HU binds primarily to the minor groove of DNA.
ISSN 09106340
Learning Resource Type Article
Publisher Date 1991-01-01
e-ISSN 13482246
Journal Analytical Sciences(analsci1985)
Volume Number 7
Issue Number Supple
Page Count 4
Starting Page 849
Ending Page 852


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