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Author Kojima, Junji ♦ Morita, Naofumi ♦ Takagi, Masanosuke
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Enzyme electrode ♦ glycerophosphate ♦ glycerophosphate oxidase ♦ peroxidase ♦ glassy carbon electrode
Abstract An enzyme sensor for L-α-glycerophosphate was prepared by covalent fixation of glycerophosphate oxidase and peroxidase by use of bovine serum albumin and glutaraldehyde onto a glassy carbon electrode silanized with 3-aminopropyl triethoxysilane. The sensor showed good linear current responses in the range of 5 to 100μM glycerophosphate (γ=0.999, n=7); the response time was about 20s. The sensor was stable for at least 60d. Sugar phosphates did not have any appreciable effect on the response of the sensor, but reducing materials such as L-ascorbic acid and L-cysteine gave some errors that changed with concentrations.
ISSN 09106340
Learning Resource Type Article
Publisher Date 1988-10-10
e-ISSN 13482246
Journal Analytical Sciences(analsci1985)
Volume Number 4
Issue Number 5
Page Count 4
Starting Page 497
Ending Page 500


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