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Author Kojima, Eijiro ♦ Kai, Masaaki ♦ Ohkura, Yosuke
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Tryptophan ♦ phenylglyoxal ♦ fluorometry ♦ serum
Abstract A simple fluorometric method is described for the determination of the total (free+albumin-bound) and free tryptophan in human serum. After eproteinization of the serum (perchloric acid precipitation for total tryptophan; ultrafiltration for free tryptophan), the samples are treated with phenylglyoxal in dilute perchloric acid to develop fluorescence. The method is sensitive and fairly selective for tryptophan in serum; the limit of detection is 0.84nmol $ml^{-1}$ in serum, thus giving a fluorescence intensity that is twice that of the blank.
ISSN 09106340
Learning Resource Type Article
Publisher Date 1993-02-10
e-ISSN 13482246
Journal Analytical Sciences(analsci1985)
Volume Number 9
Issue Number 1
Page Count 3
Starting Page 25
Ending Page 27


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