### Extraction and Isotope Measurement of Sulfur in Biological SamplesExtraction and Isotope Measurement of Sulfur in Biological Samples

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 Author Arikawa, Yoshiko ♦ Sasaki, Akira Source J-STAGE Content type Text Publisher The Japan Society for Analytical Chemistry Language English
 Subject Keyword Biosulfur ♦ biosulfur extraction ♦ combustion ♦ tin(II)-strong phosphoric acid reduction ♦ biosulfur isotopic composition Abstract A method to extract sulfur completely from biological samples was investigated to assist the isotopic measurement of biosulfur. A sample is burnt under a high pressure of oxygen. The sulfur trioxide formed is absorbed in water to fix it as barium sulfate, which in turn is heated with tin(II)-strong phosphoric acid (the reagent made by dissolving tin(II) chloride dihydrate in condensed orthophosphoric acid). The hydrogen sulfide formed by this procedure is further transformed into silver sulfide. The isotopic ratio is obtained in terms of $δ^{34}S‰$ according to the conventional method, i.e., combustion of silver sulfide with copper(I) oxide, successive vacuum distillation of formed sulfur dioxide, and measurement of its isotopic ratio by a mass spectrometer. The whole procedure was carefully examined at each stage by repeated analyses of cystine and methionine as sulfur-containing samples. Preliminary applications to measure the isotopic composition of sulfur in nail and hair samples were attempted. ISSN 09106340 Learning Resource Type Article Publisher Date 1987-04-10 e-ISSN 13482246 Journal Analytical Sciences(analsci1985) Volume Number 3 Issue Number 2 Page Count 4 Starting Page 157 Ending Page 160