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Author Ulrich, Petra ♦ Weller, Michael G. ♦ Knopp, Dietmar ♦ Niessner, Reinhard
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Humic acid ♦ enzyme-linked immunoassay ♦ polyclonal antibody ♦ cross-reactivity ♦ dissolved organic carbon ♦ water analysis
Abstract An enzyme-linked immunoassay (ELISA) for the determination of humic acid in natural-water samples was developed. For antisera production, humic acid was coupled to helix pomatia hemocyanin. A humic acid-horseradish peroxidase conjugate was synthesized as a tracer. The detection limit of this competitive assay was estimated to be 0.5μg/l, which means 100pg absolute. The cross-reactivities at the center point (50%-value) of several commercial humic acids and both aliphatic and aromatic acids were determined. Moreover, the structural aspects of the humic acids could be elucidated.
ISSN 09106340
Learning Resource Type Article
Publisher Date 1993-12-10
e-ISSN 13482246
Journal Analytical Sciences(analsci1985)
Volume Number 9
Issue Number 6
Page Count 3
Starting Page 795
Ending Page 797


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