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Author Milanovich, N. ♦ Suh, M. ♦ Jankowiak, R. ♦ Small, G. J. ♦ Hayes, J. M.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword CHEMISTRY ♦ THIAZOLES ♦ FLUORESCENCE ♦ EMISSION SPECTRA ♦ DYES ♦ ORGANIC SULFUR COMPOUNDS ♦ CHEMICAL BONDS ♦ DNA ♦ MOLECULAR STRUCTURE ♦ MOLECULES ♦ HOLES ♦ ELECTRONIC STRUCTURE
Abstract The thiazole orange derivative, TO-PRO-3, and its dimeric analogue, TOTO-3, bind strongly to both double- and single-stranded DNA. Both the bound and the free dye undergo efficient nonphotochemical hole burning. From the structure of the hole burned and the fluorescence line-narrowed spectra, it is concluded that there are multiple modes of binding of the dyes to DNA. In one mode, the absorption, fluorescence, and hole-burned vibronic band structure are similar to those of the free dye. It is proposed that the molecules in this mode are externally bound to the DNA chain. Molecules in the other binding mode are characterized by a strong interaction with DNA. These are proposed to be molecules in intercalated or base-stacked configurations. 27 refs., 7 figs., 2 tabs.
ISSN 00223654
Educational Use Research
Learning Resource Type Article
Publisher Date 1996-05-23
Publisher Place United States
Journal Journal of Physical Chemistry
Volume Number 100
Issue Number 21


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