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Author Mohammadi, Nasrin ♦ Kazemi, Bahram ♦ Farghadani, Mohammad Taghi ♦ Masoomi, Kamel ♦ Roozkhosh, Gholamreza
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Shahid Beheshti University of Medical Sciences
File Format HTM / HTML
Date Created 2015-08-01
Copyright Year ©2015
Language English
Subject Domain (in LCC) R5-920
Subject Keyword Quality and quantity of DNA ♦ Blood sample ♦ Medicine ♦ Genomic DNA extraction
Abstract Background: Extraction of blood genomicDNAis one of the main approaches for clinical and molecular biology studies. Although several methods have been developed for extraction of blood genomic DNA, most of these methods consume long time and use expensive chemicals such as proteinase K and toxic organic solvent such as phenol and chloroform. The objective of this study was to developed easy and safe method forDNAextraction from clotted and frozen whole blood. This method has many advantages: time reducing, using inexpensive materials, without phenol and chloroform, achieving of high molecular weight and good quality genomicDNA. M aterials and Methods: DNA extraction was performed by two methods (new and phenol-chloroform method). Then quantity and quality parameters were evaluated by 1% agarose gel electrophoresis, Nano drop analysis and efficiency of Polymerase Chain Reaction (PCR). R es ults: Extracted DNA from 500μL of blood samples were 457.7ng/μl and 212ng/μL and their purity (OD260/OD280) were 1.8 and 1.81 for new recommended and phenol–chloroform methods respectively. The PCR results indicated that D16S539 and CSF1PO loci were amplified. C onclusion: These results shown that this method is simple, fast, safe and most economical.
ISSN 23453346
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2015-07-01
e-ISSN 23453346
Journal Novelty in Biomedicine
Volume Number 3
Issue Number 3
Page Count 5
Starting Page 119
Ending Page 123

Source: Directory of Open Access Journals (DOAJ)