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Author Sampson, D. A. ♦ Jansen, G. R.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ DIET ♦ BIOLOGICAL EFFECTS ♦ PROTEINS ♦ BIOSYNTHESIS ♦ TRITIUM COMPOUNDS ♦ DNA ♦ LACTATION ♦ MAMMARY GLANDS ♦ MILK ♦ PHENYLALANINE ♦ PREGNANCY ♦ RATS ♦ RNA ♦ AMINO ACIDS ♦ ANIMALS ♦ BIOLOGICAL MATERIALS ♦ BODY ♦ BODY FLUIDS ♦ CARBOXYLIC ACIDS ♦ FOOD ♦ GLANDS ♦ LABELLED COMPOUNDS ♦ MAMMALS ♦ MATERIALS ♦ NUCLEIC ACIDS ♦ ORGANIC ACIDS ♦ ORGANIC COMPOUNDS ♦ ORGANS ♦ RODENTS ♦ SYNTHESIS ♦ VERTEBRATES ♦ Physiological Systems- Tracer Techniques
Abstract Protein synthesis was studied in mammary tissue of rats fed diets deficient in protein quality and/or restricted in food intake throughout gestation and lactation. Diets containing 25% wheat gluten (WG), wheat gluten plus lysine and threonine (WGLT), or casein (C) were pair-fed from conception until day 15 of lactation at 100% or 85% of WG ad libitum consumption (PF100 and PF85, respectively). A seventh group was fed C ad libitum. Rates of protein synthesis were measured in vivo at day 15 of lactation from incorporation of (3-/sup 3/H)phenylalanine. At both PF100 and PF85, fractional and absolute rates of mammary gland protein synthesis were two- to three-fold higher in rats fed C than in those fed WG. Pup weights showed similar treatment effects. Both mammary protein synthesis rates and pup weights were significantly higher in rats fed C at PF85 than rats fed WG ad libitum. Food restriction from PF100 to PF85 depressed pup weights and mammary protein synthesis rates in rats fed WGLT, but had no effect in rats fed WG. These results demonstrate that when food intake is restricted, improvement of protein quality of the maternal diet increases milk output in the rat in association with increased rates of mammary protein synthesis.
Educational Use Research
Learning Resource Type Article
Publisher Date 1985-04-01
Publisher Place United States
Journal J. Pediatr. Gastroenterol. Nutr.
Volume Number 2
Organization Colorado State Univ., Fort Collins


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