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Author Kokaram, Anil ♦ Hillebrand, Jens ♦ Ramaswami, Mani ♦ Barbee, Scott ♦ Pan, Kanyu ♦ Parker, Roy
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Frontiers Media S.A.
File Format HTM / HTML
Date Created 2014-05-22
Copyright Year ©2010
Language English
Subject Domain (in LCC) RC321-571
Subject Keyword Neuropsychiatry ♦ Biological psychiatry ♦ Neurosciences ♦ Drosophila ♦ CaMKII ♦ Me13B ♦ Synaptic mRNPs ♦ Olfactory circuitry ♦ Internal medicine ♦ Medicine ♦ Translational control
Abstract MRNP granules at adult central synapses are postulated to regulate local mRNA translation and synapse plasticity. However, they are very poorly characterized in vivo. Here, in Drosophila olfactory synapses, we present early observations and characterization of candidate synaptic mRNP particles, one of which contains a widely conserved, DEAD-box helicase, Me31B. In Drosophila, Me31B is required for translational repression of maternal and miRNA-target mRNAs. A role in neuronal translational control is primarily suggested by Me31B’s localization, in cultured primary neurons, to neuritic mRNP granules that contain: i) various translational regulators; ii) CaMKII mRNA; and iii) several P-body markers including the mRNA hydrolases, Dcp1 and Pcm/Xrn-1. In adult neurons, Me31B localizes to P-body like cytoplasmic foci/particles in neuronal soma. In addition it is present to synaptic foci that may lack RNA degradative enzymes and localize predominantly to dendritic elements of olfactory sensory and projection neurons. MARCM clones of projection-neurons mutant for Me31B show loss of both Me31B and Dcp1-positive dendritic puncta, suggesting potential interactions between these granule types. In projection neurons, expression of validated hairpin-RNAi constructs against Me31B causes visible knockdown of endogenous protein, as assessed by the brightness and number of Me31B puncta. Knockdown of Me31B also causes a substantial elevation in observed levels of a translational reporter of CaMKII, a postsynaptic protein whose mRNA has been shown to be localized to projection neuron dendrites and to be translationally regulated, at least in part through the miRNA pathway. Thus, neuronal Me31B is present in dendritic particles in vivo and is required for repression of a translationally regulated synaptic mRNA.
ISSN 16625110
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2010-11-01
e-ISSN 16625110
Journal Frontiers in Neural Circuits
Volume Number 4


Source: Directory of Open Access Journals (DOAJ)