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Author Le, Viet Thang ♦ Shigematsu, Kazuto ♦ Zha, Yan ♦ Nazneen, Arifa ♦ Razzaque, Mohammed S. ♦ Taguchi, Takashi
Source J-STAGE
Content type Text
Publisher Nagasaki University School of Medicine
Language English
Subject Keyword Obstructive nephropathy ♦ Unilateral ureteral obstruction ♦ CTGF ♦ Angiotensin II ♦ Tubulointerstitial fibrosis
Abstract Angiotensin II (Ang-II) and connective tissue growth factor (CTGF) are involved in various renal disorders that lead to end-stage renal disease. Here, we determined the role of Ang-II and CTGF in the progression of tubulointerstitial injury in the rat unilateral ureteral obstruction (UUO) model. Sprague-Dawley rats (n=16) were used; 10 rats underwent UUO, and 6 control rats underwent sham operation; and rats of both groups were sacrificed on days 7 or 14. Histomorphometric analysis was performed to quantitate tubulointerstitial injuries in the experimental group. Kidney sections were stained immunohistochemically for Ang-II, CTGF, transforming growth factor-β1 (TGF-β1), type III collagen and α-smooth muscle actin (α-SMA). Renal CTGF expression was studied using in situ hybridization and reverse transcriptase-polymerase chain reaction. Double staining for Ang-II with α-SMA and CTGF with α-SMA was performed to identify cells with enhanced expression of Ang-II and CTGF. Similar dual staining of Ang-II with type III collagen and CTGF with type III collagen was performed. The correlation between Ang-II and CTGF expression and tubulointerstitial injury was examined. In obstructed kidneys, there was a significant (p
ISSN 00016055
Learning Resource Type Article
Publisher Date 2006-01-01
Journal Acta Medica Nagasakiensia(amn)
Volume Number 51
Issue Number 1
Page Count 9
Starting Page 9
Ending Page 17


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