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Author Neselioglu, Salim ♦ Ergin, Merve ♦ Erel, Ozcan
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Acetohydroxamic acid ♦ ceruloplasmin ♦ copper ♦ ferroxidase
Abstract A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2017-12-10
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 33
Issue Number 12
Page Count 6
Starting Page 1339
Ending Page 1344


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