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Author Bekdeser, Burcu ♦ Özyürek, Mustafa ♦ Akyüz, Esin ♦ Apak, Resat
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Peroxynitrite anion ♦ gentisic acid ♦ spectrofluorometric method ♦ Pyrogallol Red bleaching method
Abstract In this study, a novel fluorometric method for the determination of peroxynitrite anion $(ONOO^{–})$ scavenging (PAS) activity of amino acids and biothiols, which can mostly trap peroxynitrite in vivo, is described. This assay is based on the conversion of a gentisic acid probe to its non-fluorescent oxidation products with $ONOO^{–}.$ The attenuation of the fluorescence intensity (FI) of the probe upon peroxynitrite attack is diminished with antioxidants, the difference in FI being related to the PAS activity of the antioxidants. The $IC_{50}$ (50% inhibitive concentration) values of biothiols, amino acids and tissue homogenates were estimated, in comparison with the reference Pyrogallol Red (PR) bleaching method. PR is the most suitable and frequently used dye to determine PAS activity, but is relatively insensitive. The developed fluorometric assay is highly sensitive to allow determinations of the PAS activity of amino acids.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2016-12-10
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 32
Issue Number 12
Page Count 6
Starting Page 1315
Ending Page 1320


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