Thumbnail
Access Restriction
Open

Author Lu, Jian ♦ Zhou, Zhongping ♦ Zheng, Jianzhou ♦ Zhang, Zhuyi ♦ Lu, Rongzhu ♦ Liu, Hanqing ♦ Shi, Haifeng ♦ Tu, Zhigang
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CADMIUM ♦ CARCINOGENS ♦ CELL TRANSFORMATIONS ♦ DNA ♦ DNA REPAIR ♦ HEAVY METALS ♦ LARGE INTESTINE ♦ MITOCHONDRIA ♦ PROTEINS ♦ TOXICITY ♦ TRANSCRIPTION
Abstract Cadmium is a toxic heavy metal present in the environment and in industrial materials. Cadmium has demonstrated carcinogenic activity that induces cell transformation, but how this occurs is unclear. We used 2D-DIGE and MALDI TOF/TOF MS combined with bioinformatics and immunoblotting to investigate the molecular mechanism of cadmium transformation. We found that small GTPases were critical for transformation. Additionally, proteins involved in mitochondrial transcription, DNA repair, and translation also had altered expression patterns in cadmium treated cells. Collectively, our results suggest that activation of small GTPases contributes to cadmium-induced transformation of colon cells. - Highlights: • Colon epithelial cell line is firstly successfully transformed by cadmium. • 2D-DIGE is applied to visualize the differentially expressed proteins. • RhoA plays an important role in cadmium induced malignant transformation. • Bioinformatic and experimental methods are combined to explore new mechanisms.
ISSN 0041008X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-10-01
Publisher Place United States
Journal Toxicology and Applied Pharmacology
Volume Number 288
Issue Number 1


Open content in new tab

   Open content in new tab