Thumbnail
Access Restriction
Open

Author Takeda, Tomoki ♦ Taura, Junki ♦ Hattori, Yukiko ♦ Ishii, Yuji ♦ Yamada, Hideyuki
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ DIOXIN ♦ FETUSES ♦ FSH ♦ GROWTH FACTORS ♦ HYDROCARBONS ♦ INSULIN ♦ LIBERINS ♦ LITTER SIZE ♦ LUTEINIZING HORMONE ♦ MESSENGER-RNA ♦ MICE ♦ NEONATES ♦ OVARIES ♦ PLANT GROWTH ♦ RATS ♦ RECEPTORS ♦ STH ♦ TESTES ♦ TSH
Abstract We have previously revealed that treating pregnant rats with 2,3,7,8-tetracholorodibenzo-p-dioxin (TCDD) reduces the expression of gonadotropins and growth hormone (GH) in the fetal and neonatal pituitary. A change in gonadotropin expression impairs the testicular expression of steroidogenic proteins in perinatal pups, and imprint defects in sexual behavior after reaching maturity. In this study, we examined whether TCDD also affects the expression of gonadotropin and GH in mice using C57BL/6J and DBA/2J strains which express the aryl hydrocarbon receptor (Ahr) exhibiting a different affinity for TCDD. When pregnant C57BL/6J mice at gestational day (GD) 12 were given oral TCDD (0.2–20 μg/kg), all doses significantly attenuated the pituitary expression of gonadotropin mRNAs in fetuses at GD18. On the other hand, in DBA/2J mice, a much higher dose of TCDD (20 μg/kg) was needed to produce a significant attenuation. Such reduction in the C57BL/6J strain continued until at least postnatal day (PND) 4. In agreement with this, TCDD reduced the testicular expression of steroidogenic proteins in C57BL/6J neonates at PND2 and 4, although the same did not occur in the fetal testis and ovary. Furthermore, TCDD reduced the perinatal expression of GH, litter size and the body weight of newborn pups only in the C57BL/6J strain. These results suggest that 1) also in mice, maternal exposure to TCDD attenuates gonadotropin-regulated steroidogenesis and GH expression leading to the impairment of pup development and sexual immaturity; and 2) Ahr activation during the late fetal and early postnatal stages is required for these defects. - Highlights: • The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on mouse growth was studied. • TCDD reduced the levels of luteinizing hormone and growth hormone in perinatal pups. • Maternal exposure to TCDD also attenuated testicular steroidogenesis in pups. • The above effects of TCDD were more pronounced in C57BL/6J than in DBA/2J strain. • TCDD seems to disturb pup maturation by activating aryl hydrocarbon receptor.
ISSN 0041008X
Educational Use Research
Learning Resource Type Article
Publisher Date 2014-08-01
Publisher Place United States
Journal Toxicology and Applied Pharmacology
Volume Number 278
Issue Number 3


Open content in new tab

   Open content in new tab