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Author Yamashita, Shuya ♦ Baba, Kiwako ♦ Makio, Akiko ♦ Kumazoe, Motofumi ♦ Huang, Yuhui ♦ Lin, I.-Chian ♦ Bae, Jaehoon ♦ Murata, Motoki ♦ Yamada, Shuhei ♦ Tachibana, Hirofumi
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ AMINO ACIDS ♦ CHOLESTEROL ♦ DNA ♦ FOOD ♦ GENES ♦ HYDROCARBONS ♦ LIGANDS ♦ MELANOMAS ♦ MICE ♦ MINERALS ♦ MOLECULES ♦ PLANTS ♦ POLYPHENOLS ♦ RECEPTORS ♦ SENSITIVITY ♦ VITAMIN E
Abstract Previous studies have identified biomolecules that mediate the physiological actions of food factors, such as amino acids, vitamins, fatty acids, minerals, plant polyphenols, and lactobacilli, suggesting that our bodies are equipped with an innate system that senses which food factors are required to maintain our health. However, the effects of environmental factors on food factor sensing (FFS) remains largely unknown. Tocotorienols (T3s), which belongs to the vitamin E family, possess several physiological functions, including cholesterol lowering and neuroprotective effects. Here, we investigated the effects of naturally abundant γ-T3 on FFS-related gene expressions in melanoma using a DNA chip. Our results showed that γ-T3 increased the expression level of aryl hydrocarbon receptor (AhR), a sensing molecule to plant polyphenol baicalein. The co-treatment with γ-T3 and baicalein enhanced the anti-proliferative activity of baicalein, accompanied by the downstream events of AhR-activation induced by baicalein. These data suggest that γ-T3 upregulates AhR expression and enhances its sensitivity to baicalein. - Highlights: • γ-T3 upregulated the expression of AhR in mouse melanoma. • Promotion of the binding activity of Sp1 is associated with the increasing effect of γ-T3 on AhR expression. • γ-T3 enhanced the anti-proliferative activity of baicalein that has an AhR ligand activity. • γ-T3 enhanced the inducing activity of baicalein on the expression of AhR target genes.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-13
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 473
Issue Number 4


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