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Author Wijnen, Bas ♦ Petersen, Emily E. ♦ Hunt, Emily J. ♦ Pearce, Joshua
Source Hyper Articles en Ligne (HAL)
Content type Text
Publisher Wiley
File Format PDF
Language English
Subject Keyword open hardware ♦ stage ♦ microscope ♦ optics ♦ imaging ♦ 3-D microscope ♦ info ♦ spi ♦ Computer Science [cs]/Databases [cs.DB] ♦ Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Abstract Open-source technology not only has facilitated the expansion of the greater research community, but by lowering costs it has encouraged innovation and customizable design. The field of automated microscopy has continued to be a challenge in accessibility due the expense and inflexible, non-interchangeable stages. This paper presents a low-cost, open source microscope 3-D stage. A RepRap 3-D printer was converted to an optical microscope equipped with a customized, 3-D printed holder for a USB microscope. Precision measurements were determined to have an average error of 10 μm at the maximum speed and 27 μm at the minimum recorded speed. Accuracy tests yielded an error of 0.15%. The machine is a true 3-D stage and thus able to operate with USB microscopes or conventional desktop microscopes. It is larger than all commercial alternatives, and is thus capable of high depth images over unprecedented areas and complex geometries. The repeatibility is below 2-D microscope stages, but testing shows that it is adequate for the majority of scientific applications. The open source microscope stage costs less than 3% to 9% of the closest proprietary commercial stages. This extreme affordability vastly improves accessibility for 3-D microscopy throughout the world.
ISSN 00222720
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-11-01
e-ISSN 13652818
Journal Journal of Microscopy
Volume Number 264
Issue Number 2
Page Count 9
Starting Page 238
Ending Page 246