|Author||Kurtides, E. S. ♦ Rambach, W. A. ♦ Alt, H. L. ♦ Wurster, J. C.|
|Source||United States Department of Energy Office of Scientific and Technical Information|
|Subject Keyword||BIOLOGY AND MEDICINE ♦ AMINO ACIDS ♦ ANEMIA ♦ BIOCHEMISTRY ♦ BLOOD CELLS ♦ BLOOD FORMATION ♦ BLOOD PLASMA ♦ BODY ♦ ERYTHROPOIETIN ♦ HORMONES ♦ METABOLISM ♦ NUCLEIC ACIDS ♦ OXYGEN ♦ PROTEINS ♦ RABBITS ♦ RADIATION DOSES ♦ RADIATION PROTECTION ♦ RATS ♦ SPLEEN ♦ THYMIDINE ♦ TRACER TECHNIQUES ♦ TRITIUM ♦ WEIGHT ♦ X RADIATION|
|Abstract||The action of erythropoiesis-stimulating factor (ESF, erythropoietin) obtalned from the plasma of anemic rabbits was demonstrated by measuring the uptake of H/sup 3/-labeled thymidine by rat spleen. The splenic incorporation of thymidine in ESF-stimulated animals was significantly elevated over control values at all time intervals studied (15 to 180 min). Maximum labeling occurred at 60 min, with the 180-min values being essentially equal to those obtained at 60 min. The values reflect tissue proliferative activity since under the stimulus of ESF, thymidine uptake is increased by a factor of 4. The 60-min values most directly reflect DNA incorporation of label and indicate the DNA synthetic capacity of tissues. In order to study the effect of ESF on cells of the reticuloendothelial system (RES) in spleen, the animals were made hypoxic (maintained in a 7.7% O atmosphere) and irradiated (800 r, whole-body 220-kv x rays). Acute hynoxia was shown to protect against x radiation. Although the absolute number of splenic RES cells was not increased by irradiation, their relative counts in spleen increased 5-fold 96 hr after irradiation. Spleen weight decreased 36 to 46% and was not affected by injection of ESF. Body weight and hematocrit remained normal but circulating reticulocyte count fell after irradiation and was not restored by ESF. ESF injection also failed to stimulate the incorporation of thymidineH/sup 3/ in RES cells, indicating its selective effect on hemopoietic cells. (H.H.D.)|
|Learning Resource Type||Article|
|Journal||Journal of Laboratory and Clinical Medicine|
|Organization||Northwestern Univ., Chicago|
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
For any issue or feedback, please write to email@example.com