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Author Voordouw, G. ♦ Shen, Y. ♦ Teland, A. J. ♦ Jack, T. R. ♦ Westlake, W. S.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ PETROLEUM ♦ DNA ♦ EVALUATION ♦ MICROORGANISMS ♦ GENES ♦ OIL FIELDS ♦ PATTERN RECOGNITION ♦ PROBES ♦ GEOLOGIC DEPOSITS ♦ MINERAL RESOURCES ♦ NUCLEIC ACIDS ♦ ORGANIC COMPOUNDS ♦ PETROLEUM DEPOSITS ♦ RESOURCES 560300* -- Chemicals Metabolism & Toxicology ♦ Petroleum
Abstract This paper presents a protocol for quantitative analysis of microbial communities by reverse sample genome probing is presented in which (i) whole community DNA is isolated and labeled in the presence of a known amount of an added internal standard and (ii) the resulting spiked reverse genome probe is hybridized with a master filter on which denatured genomic DNAs from bacterial standards isolated from the target environment were spotted in large amounts (up to 1,500 ng) in order to improve detection sensitivity. This protocol allowed reproducible fingerprinting of the microbial community in oil field production waters at 19 sites from which water and biofilm samples were collected. It appeared that selected sulfate-reducing bacteria were significantly enhanced in biofilms covering the metal surfaces in contact with the production waters.
ISSN 00992240
Educational Use Research
Learning Resource Type Article
Publisher Date 1993-12-01
Publisher Place United States
Journal Applied and Environmental Microbiology
Volume Number 59
Issue Number 12


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