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Author PATTEN, RAYMOND A. ♦ GORDY, WALTER
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Publisher Nature Publishing Group
Language English
Subject Keyword CHEMISTRY ♦ ABSORPTION ♦ ATOMS ♦ BIOCHEMISTRY ♦ BIOLOGY ♦ CATIONS ♦ COBALT 60 ♦ COMPLEXES ♦ DIAGRAMS ♦ ELECTRON SPIN RESONANCE ♦ ELECTRONS ♦ FREE RADICALS ♦ FREQUENCY ♦ GAMMA RADIATION ♦ GAMMA SOURCES ♦ HIGH TEMPERATURE ♦ HYDROGEN ♦ IRRADIATION ♦ LOW TEMPERATURE ♦ MAGNETIC RESONANCE ♦ MANGANESE COMPOUNDS ♦ MEASURED VALUES ♦ MIXING ♦ PRODUCTION ♦ RADIATION CHEMISTRY ♦ RADIATION DOSES ♦ RADIOBIOLOGY ♦ RESONANCE ♦ RIBONUCLEIC ACID ♦ RNA ♦ SPIN ♦ TEMPERATURE ♦ VARIATIONS ♦ VESSELS ♦ WATER
Abstract Samples of DNA and RNA were irradiated with a 700 deg C Co/sup 60/ source directly in an absorption cell, which was maintained at a temperature of 4.2 deg K. Dosages of the order of 10/sup 6/ rads were given. Electron spin resonance observations were made at the same temperature, at a frequency of 23 kMc. The electron spin resonance signals for pure DNA and RNA (with no water) are similar to those found at room temperature, only much stronger for the same dosage of radiation. When the samples irradiated at 4.2 deg K were allowed to warm to room temperature, spinrecombination reduced the electron spin resonance signal to that obtained after irradiation of the samples with the same dosage at room temperature. A marked decrease in the formation of free radicals from DNA and RNA was found when the water concentration is increased above 50% (by weight). The effects of water indicate transfer of energy from the nucleic acid to the water matrix. Hydrogen-atom and Mn/sup 2+/ resonance in the nucleic acidwater complex are discussed. (P.C.H.)
ISSN 00280836
Educational Use Research
Learning Resource Type Article
Publisher Date 1964-01-25
Publisher Department Duke Univ., Durham, N.C.
Journal Nature
Volume Number 201
Issue Number 4917
Organization Duke Univ., Durham, N.C.


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