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Author Saito, Yuki ♦ Iwatsuki, Ken ♦ Hanyu, Hikaru ♦ Maruyama, Natsuki ♦ Aihara, Eitaro ♦ Tadaishi, Miki ♦ Shimizu, Makoto ♦ Kobayashi-Hattori, Kazuo
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CELL DIFFERENTIATION ♦ CELL PROLIFERATION ♦ CULTURE MEDIA ♦ GOLGI COMPLEXES ♦ METHIONINE ♦ SMALL INTESTINE ♦ STEM CELLS
Abstract We investigated the effects of essential amino acids on intestinal stem cell proliferation and differentiation using murine small intestinal organoids (enteroids) from the jejunum. By selectively removing individual essential amino acids from culture medium, we found that 24 h of methionine (Met) deprivation markedly suppressed cell proliferation in enteroids. This effect was rescued when enteroids cultured in Met deprivation media for 12 h were transferred to complete medium, suggesting that Met plays an important role in enteroid cell proliferation. In addition, mRNA levels of the stem cell marker leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) decreased in enteroids grown in Met deprivation conditions. Consistent with this observation, Met deprivation also attenuated Lgr5-EGFP fluorescence intensity in enteroids. In contrast, Met deprivation enhanced mRNA levels of the enteroendocrine cell marker chromogranin A (ChgA) and markers of K cells, enterochromaffin cells, goblet cells, and Paneth cells. Immunofluorescence experiments demonstrated that Met deprivation led to an increase in the number of ChgA-positive cells. These results suggest that Met deprivation suppresses stem cell proliferation, thereby promoting differentiation. In conclusion, Met is an important nutrient in the maintenance of intestinal stem cells and Met deprivation potentially affects cell differentiation. - Highlights: • Met influences the proliferation of enteroids. • Met plays a crucial role in the maintenance of stem cells. • Met deprivation potentially promotes differentiation into secretory cells.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-06-17
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 488
Issue Number 1


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