Thumbnail
Access Restriction
Open

Author Wei, Qin ♦ Wu, Dan ♦ Li, Yan ♦ Du, Bin ♦ Wang, Jun Hong
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract A simple and sensitive method was conducted for the determination of trace amounts of proteins with benzeneazo-8-acetylamino-1-naphthol-3,6-disulfonic acid sodium salt (azophloxine, AP) using a Rayleigh light-scattering (RLS) technique. At pH 2.60 and in the presence of an emulsifier OP microemulsion, the RLS of AP can be greatly enhanced by proteins, owing to the interaction between AP and protein. The enhanced intensity is proportional to the concentration of proteins. Four proteins, including bovine serum albumin (BSA), human serum albumin (HSA), lysozyme (Lys) and γ-globulin (γ-G) have been tested. For example, the linear range of BSA was 0 - 0.06 µg $mL^{-1}$ with detection limits of 2.38 ng $mL^{-1}.$ The method was applied to the analysis of protein in human urine and penicillin samples with satisfactory results. The relative standard deviation was in all instances less than 4.0%, and the recovery was in the range of 97.5 - 104%.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2006-01-01
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 22
Issue Number 2
Page Count 5
Starting Page 275
Ending Page 279


Open content in new tab

   Open content in new tab