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Author Kameda, Tsunenori ♦ Ishii, Tadashi ♦ Matsunaga, Toshiro ♦ Ashida, Jun
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract The boron in plant cell walls, which is water-insoluble and in the solid state, is solubilized by pectinase digestion to give a dimeric rhamnogalacturonan II-borate (dRG-II-B) complex. To clarify the nondestructive structure of boron present in plant cell walls (as represented by sugar beet fiber), we performed 192- and 96-MHz $^{11}B$ solid state NMR measurements. The use of a high field magnet frequency of 192-MHz enabled us to observe $^{11}B$ isotropic chemical shifts at -9.7 and -9.6 ppm for dRG-II-B and sugar beet fiber in the solid state, respectively, demonstrating that the boron in isolated dRG-II-B and in plant cell walls is present as a borate-diol ester (1:2). The observation of the magnetic field dependence of the chemical shift and lineshape for the borate-diol ester (1:2) by quadrupolar interaction suggested that the borate complex had a distorted tetrahedral boron structure.
ISSN 09106340
Learning Resource Type Notes
Publisher Date 2006-01-01
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 22
Issue Number 2
Page Count 3
Starting Page 321
Ending Page 323


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