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Author Watanabe, Jun ♦ Oki, Tomoyuki ♦ Takebayashi, Jun ♦ Yamasaki, Koji ♦ TAKANO-ISHIKAWA, Yuko ♦ Hino, Akihiro ♦ Yasui, Akemi
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract Hydrophilic oxygen radical absorbance capacity (H-ORAC) is a method for evaluating antioxidant capacities of solutions of hydrophilic compounds. In this study, we improved the original method for H-ORAC determination, and evaluated the precision of the two improved methods (methods A and B) by interlaboratory studies using 5 antioxidant solutions and 5 food extracts as test samples. An interlaboratory study of method A, in accordance with the harmonized protocol, demonstrated satisfactory performance (intermediate precision relative standard deviations $(RSD_{int})$ ranging from 4.6 to 18.8%; the reproducibility relative standard deviations $(RSD_{R})$ ranging from 7.0 to 21.1%, and the HorRat values ranging from 0.40 to 1.93). However, methodological problems remained, and a further improved method, method B, was thus developed. An interlaboratory study of method B by 5 participating laboratories showed better intermediate precision and reproducibility $(RSD_{int}$ and $RSD_{R}$ ranging from 1.8 to 9.4%, and from 4.4 to 13.8%, respectively), and all HorRat values for the test samples were less than 1.3, suggesting good performance for the H-ORAC measurement.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2012-02-10
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 28
Issue Number 2
Page Count 1
Starting Page 159
Ending Page 159


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