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Author Zhu, Chang-Qing ♦ Wu, Yu-Qin ♦ Zheng, Hong ♦ Chen, Jin-Long ♦ Li, Dong-Hui ♦ Li, Shun-Hua ♦ Xu, Jin-Gou
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract A near-infrared (near-IR) fluorescence quenching method was developed for the determination of nucleic acids in aqueous solution by using a cationic heptamethylene thiacyanine as a probe. The near-IR cationic cyanine showed maximum excitation and emission wavelengths at 800 and 825 nm, respectively, in the presence of Triton X-100; the fluorescence of the cyanine could be greatly quenched by DNA. The calibration graphs were linear over the range of 10 - 400 ng/mL for CT (calf thymus) DNA and over the range 5 - 400 ng/mL for FS (fish sperm) DNA under optimal conditions. The corresponding detection limits were 5.2 ng/mL for CT DNA and 2.5 ng/mL for FS DNA. The relative standard deviation (n = 8) was 3.1% for 75 ng/mL CT DNA and 2.2% for 75 ng/mL FS DNA, respectively. Preliminary research showed that the fluorescence quenching might be ascribed to the formation of dye aggregate facilitated by DNA.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2004-01-01
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 20
Issue Number 6
Page Count 5
Starting Page 945
Ending Page 949


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