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Author Matsunaga, Toshiro ♦ Moriizumi, Mihoko ♦ Kameda, Tsunenori
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Subject Keyword Organic nitrogen ♦ cattle manure compost ♦ humic substance ♦ size-exclusion HPLC ♦ chemiluminescent nitrogen detection
Abstract To characterize organic nitrogen present in cattle manure compost, a hot-water extract of the compost along with an acid-insoluble (humic acid-like) fraction either treated with alkali or laccase or untreated were separated by size-exclusion high-performance liquid chromatography (SE-HPLC). Nitrogen was then detected by chemiluminescent nitrogen detection (CLND), and humic acid-like substances were detected by measuring the absorbance at 420 nm. The acid-insoluble fraction comprised the higher molecular-weight region in the chromatogram of the whole extract, and its chromatogram pattern of CLND was similar to that of the absorbance at 420 nm. Changes in the molecular-weight distribution of the acid-insoluble fraction detected by CLND due to alkaline degradation and laccase polymerization were in agreement with those observed by measuring the absorbance at 420 nm. These results, together with other data, demonstrate that protein and humic acid-like moieties are present in the same molecule in compost organic matter.
ISSN 09106340
Learning Resource Type Notes
Publisher Date 2013-09-10
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 29
Issue Number 9
Page Count 4
Starting Page 923
Ending Page 926


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