Thumbnail
Access Restriction
Open

Author Zhang, Li ♦ Li, Na ♦ Zhao, Fenglin ♦ Li, Ke’an
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract The interaction of Methylene Blue (MB) with chondroitin-4-sulfate (CHS) has been investigated using spectroscopic techniques, including UV-Vis absorption, Rayleigh resonance scattering (RRS), and circular dichroism (CD). The addition of CHS caused a decrease in the absorbance of MB at 664 nm with a new absorption band appearing at 570 nm, enhanced RRS at 314 nm and 560 nm, and also resulted in an intense CD signal at 568 nm. The Scatchard model has been applied to calculating the binding constant and the number of binding sites. The calculated parameters are consistent with the experimental results. The factors affecting the interaction were investigated. Quantitative spectroscopic methods were developed for the first time. They are based on the fact that a decrease in the absorption at 664 nm and an enhancement of the RRS intensity at 314 nm are proportional to the concentration of CHS added in a certain range. Satisfactory results were obtained on the determination of synthetic samples.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2004-01-01
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 20
Issue Number 3
Page Count 6
Starting Page 445
Ending Page 450


Open content in new tab

   Open content in new tab