Development and Validation of a Liquid Chromatographic Method for the Analysis of Positron Emission Tomography Radiopharmaceuticals with $Ru(bpy)_{3}^{2+}-KMnO_{4}$ Chemiluminescence DetectionDevelopment and Validation of a Liquid Chromatographic Method for the Analysis of Positron Emission Tomography Radiopharmaceuticals with $Ru(bpy)_{3}^{2+}-KMnO_{4}$ Chemiluminescence Detection

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 Author Nakao, Ryuji ♦ Furutsuka, Kenji ♦ Yamaguchi, Masatoshi ♦ Suzuki, Kazutoshi Source J-STAGE Content type Text Publisher The Japan Society for Analytical Chemistry Language English
 Abstract A sensitive, selective and rapid high-performance liquid chromatographic (HPLC) method with chemiluminescence (CL) detection was developed and validated for the analysis of positron emission tomography (PET) radiopharmaceuticals. This method is based on the CL reaction of PET compounds with tris(2,2′-bipyridyl)ruthenium(II) $[Ru(bpy)_{3}^{2+}]$ and acidic potassium permanganate $[KMnO_{4}].$ After optimization of the reaction conditions, 12 of the 14 PET compounds investigated could be successfully detected and showed good performance in terms of sensitivity, linearity and reproducibility. In particular, for compounds with a tertiary amine functional group, the limits of detection were ppb levels for a 20 µL injection volume. Finally, this method was used to determine PET compounds for calculating of specific radioactivity in pharmaceutical samples. ISSN 09106340 Learning Resource Type Article Publisher Date 2007-01-01 e-ISSN 13482246 Journal Analytical Sciences(analsci) Volume Number 23 Issue Number 2 Page Count 5 Starting Page 151 Ending Page 155