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Author Mizuno, Hajime ♦ Tsuyama, Naohiro ♦ Date, Sachiko ♦ Harada, Takanori ♦ Masujima, Tsutomu
Source J-STAGE
Content type Text
Publisher The Japan Society for Analytical Chemistry
Language English
Abstract A direct and rapid metabolic analysis of a live single cell was performed by live single-cell video-mass spectrometry. The contents of the cytoplasm and a granule were sucked into a nano-electrospray ionization (nano-ESI) tip, and were directly introduced into a Q-TOF mass spectrometer by nano-spray after the addition of an ionization solvent. The metabolic pathways and the locations of tryptophan and histidine metabolites were traced by this method for a cultured rat basophil leukemia cell line (RBL-2H3). The t-values of detected peaks by a t-test between the different location, e.g. cytoplasm and a granule, revealed the molecular localization of each MS peak. A direct and quick metabolomic analysis of a living cell under simultaneous video-microscopic observations was innovated.
ISSN 09106340
Learning Resource Type Article
Publisher Date 2008-12-10
e-ISSN 13482246
Journal Analytical Sciences(analsci)
Volume Number 24
Issue Number 12
Page Count 3
Starting Page 1525
Ending Page 1527


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