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Author Li, Qiong ♦ Gluch, Jürgen ♦ Krüger, Peter ♦ Gall, Martin ♦ Neinhuis, Christoph ♦ Zschech, Ehrenfried
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ BIOMEDICAL RADIOGRAPHY ♦ HARD X RADIATION ♦ IMAGES ♦ PINES ♦ PLANT BREEDING ♦ POLLEN ♦ SCANNING ELECTRON MICROSCOPY ♦ SPECIES DIVERSITY ♦ THREE-DIMENSIONAL CALCULATIONS
Abstract A laboratory-based X-ray microscope is used to investigate the 3D structure of unstained whole pollen grains. For the first time, high-resolution laboratory-based hard X-ray microscopy is applied to study pollen grains. Based on the efficient acquisition of statistically relevant information-rich images using Zernike phase contrast, both surface- and internal structures of pine pollen - including exine, intine and cellular structures - are clearly visualized. The specific volumes of these structures are calculated from the tomographic data. The systematic three-dimensional study of pollen grains provides morphological and structural information about taxonomic characters that are essential in palynology. Such studies have a direct impact on disciplines such as forestry, agriculture, horticulture, plant breeding and biodiversity. - Highlights: • The unstained whole pine pollen was visualized by high-resolution laboratory-based HXRM for the first time. • The comparison study of pollen grains by LM, SEM and high-resolution laboratory-based HXRM. • Phase contrast imaging provides significantly higher contrast of the raw images compared to absorption contrast imaging. • Surface and internal structure of the pine pollen including exine, intine and cellular structures are clearly visualized. • 3D volume data of unstained whole pollen grains are acquired and the specific volumes of the different layer are calculated.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-10-14
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 479
Issue Number 2


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