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Author Inukai, Yoshiaki ♦ Sakamoto, Tomoaki ♦ Ueguchi-Tanaka, Miyako ♦ Shibata, Yohko ♦ Gomi, Kenji ♦ Umemura, Iichiro ♦ Hasegawa, Yasuko ♦ Ashikari, Motoyuki ♦ Kitano, Hidemi ♦ Matsuoka, Makoto
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Plant Structures ♦ Anatomy ♦ Heterocyclic Compounds ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Gene Expression Regulation, Plant ♦ Genetics ♦ Indoleacetic Acids ♦ Metabolism ♦ Oryza Sativa ♦ Growth & Development ♦ Plant Proteins ♦ Plant Roots ♦ Transcription Factors ♦ Amino Acid Motifs ♦ Drug Effects ♦ Pharmacology ♦ Molecular Sequence Data ♦ Promoter Regions, Genetic ♦ Response Elements ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Although the importance of auxin in root development is well known, the molecular mechanisms involved are still unknown. We characterized a rice (Oryza sativa) mutant defective in crown root formation, crown rootless1 (crl1). The crl1 mutant showed additional auxin-related abnormal phenotypic traits in the roots, such as decreased lateral root number, auxin insensitivity in lateral root formation, and impaired root gravitropism, whereas no abnormal phenotypic traits were observed in aboveground organs. Expression of Crl1, which encodes a member of the plant-specific ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES protein family, was localized in tissues where crown and lateral roots are initiated and overlapped with beta-glucuronidase staining controlled by the DR5 promoter. Exogenous auxin treatment induced Crl1 expression without de novo protein biosynthesis, and this induction required the degradation of AUXIN/INDOLE-3-ACETIC ACID proteins. Crl1 contains two putative auxin response elements (AuxREs) in its promoter region. The proximal AuxRE specifically interacted with a rice AUXIN RESPONSE FACTOR (ARF) and acted as a cis-motif for Crl1 expression. We conclude that Crl1 encodes a positive regulator for crown and lateral root formation and that its expression is directly regulated by an ARF in the auxin signaling pathway.
Description Country affiliation: Japan
Author Affiliation: Inukai Y ( Bioscience and Biotechnology Center, Nagoya University, Nagoya, Aichi 464-8601, Japan.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2005-05-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 17
Issue Number 5


Source: WHO-Global Index Medicus