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Author Hou, Xiaomin ♦ Li, Chunsheng ♦ Gu, Wei ♦ Guo, Zhijun ♦ Yin, Wenpeng ♦ Zhang, Da
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Eukaryota ♦ Organisms ♦ Nervous System Diseases ♦ Cardiovascular Diseases ♦ Diseases ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Complex Mixtures ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Therapeutics ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Biological Sciences
Subject Keyword Discipline Emergency ♦ Discipline Medicine ♦ Brain Edema ♦ Prevention & Control ♦ Cytokines ♦ Blood ♦ Drugs, Chinese Herbal ♦ Therapeutic Use ♦ Heart Arrest ♦ Drug Therapy ♦ Neuroprotective Agents ♦ Animals ♦ Aquaporin 4 ♦ Analysis ♦ Brain Chemistry ♦ Drug Effects ♦ Etiology ♦ Complications ♦ Physiopathology ♦ Injections, Intravenous ♦ Interleukin-6 ♦ Interleukins ♦ Matrix Metalloproteinase 9 ♦ Nf-kappa B ♦ Real-time Polymerase Chain Reaction ♦ Swine ♦ Tumor Necrosis Factor-alpha ♦ Journal Article
Abstract OBJECTIVE: Shenfu injection (SFI), a traditional Chinese formulation, has been confirmed to be protective against brain during ischemia and reperfusion injury. In this exploratory study, we investigated the action of SFI in regulating the inflammatory response and brain edema after cardiopulmonary resuscitation. METHODS: After 8 minutes of untreated ventricular fibrillation (VF), pigs in the cardiopulmonary resuscitation group (n = 24) received a central venous injection of either SFI (SFI group; 1.0 mL/kg), epinephrine (EP group; 0.02 mg/kg), or saline (SA group). Levels of porcine-specific tumor necrosis factor and interleukin in sera were measured using enzyme-linked immunosorbent assay at 0.5, 1, 2, 4, 6, and 24 hours after return of spontaneous circulation (ROSC). Surviving pigs were killed 24 hours after ROSC, and the brains were removed for electron microscopy, Western blotting, and quantitative real-time polymerase chain reaction analysis. RESULTS: Compared with the EP and SA groups, SFI decreased the levels of tumor necrosis factor and interleukin-6 in serum and the brain (P < .05) and decreased the expression of nuclear factor κB and aquaporin-4 messenger RNA in the brain (P < .05). Shenfu injection also inhibited the expression of nuclear factor κB, matrix metalloproteinase 9, and aquaporin-4 protein after ROSC (P < .05). Observation of brain tissue ultrastructure showed that injury was alleviated in the SFI group compared with the SA and EP groups. CONCLUSIONS: Our exploratory experiments demonstrated that SFI reduced cerebral damage in a porcine model of VF, which may be related to suppression of the inflammatory reaction and decreased brain edema after ROSC.
Description Country affiliation: China
Author Affiliation: Hou X ( Emergency Department of Beijing Chaoyang Hospital, Capital Medical University, Chaoyang District, Beijing, China.)
ISSN 07356757
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-08-01
Publisher Place United States
e-ISSN 15328171
Journal The American Journal of Emergency Medicine
Volume Number 31
Issue Number 8


Source: WHO-Global Index Medicus