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Author Feraru, Elena ♦ Feraru, Mugurel I. ♦ Asaoka, Rin ♦ Paciorek, Tomasz ♦ De Rycke, Riet ♦ Tanaka, Hirokazu ♦ Nakano, Akihiko ♦ Friml, Jirí
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Metabolism ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Arabidopsis Proteins ♦ Metabolism ♦ Arabidopsis ♦ Cell Membrane ♦ Endosomes ♦ Trans-golgi Network ♦ Drug Effects ♦ Genetics ♦ Ultrastructure ♦ Brefeldin A ♦ Pharmacology ♦ Gtp-binding Proteins ♦ Green Fluorescent Proteins ♦ Membrane Transport Proteins ♦ Mutation, Missense ♦ Plant Roots ♦ Plants, Genetically Modified ♦ Protein Transport ♦ Seedling ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Constitutive endocytic recycling is a crucial mechanism allowing regulation of the activity of proteins at the plasma membrane and for rapid changes in their localization, as demonstrated in plants for PIN-FORMED (PIN) proteins, the auxin transporters. To identify novel molecular components of endocytic recycling, mainly exocytosis, we designed a PIN1-green fluorescent protein fluorescence imaging-based forward genetic screen for Arabidopsis thaliana mutants that showed increased intracellular accumulation of cargos in response to the trafficking inhibitor brefeldin A (BFA). We identified bex5 (for BFA-visualized exocytic trafficking defective), a novel dominant mutant carrying a missense mutation that disrupts a conserved sequence motif of the small GTPase, RAS GENES FROM RAT BRAINA1b. bex5 displays defects such as enhanced protein accumulation in abnormal BFA compartments, aberrant endosomes, and defective exocytosis and transcytosis. BEX5/RabA1b localizes to trans-Golgi network/early endosomes (TGN/EE) and acts on distinct trafficking processes like those regulated by GTP exchange factors on ADP-ribosylation factors GNOM-LIKE1 and HOPM INTERACTOR7/BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE1, which regulate trafficking at the Golgi apparatus and TGN/EE, respectively. All together, this study identifies Arabidopsis BEX5/RabA1b as a novel regulator of protein trafficking from a TGN/EE compartment to the plasma membrane.
Description Country affiliation: Belgium
Author Affiliation: Feraru E ( Department of Plant Systems Biology, VIB, and Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-07-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 24
Issue Number 7

Source: WHO-Global Index Medicus