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Author Mortimer, Jenny C. ♦ Yu, Xiaolan ♦ Albrecht, Sandra ♦ Sicilia, Francesca ♦ Huichalaf, Mariela ♦ Ampuero, Diego ♦ Michaelson, Louise V. ♦ Murphy, Alex M. ♦ Matsunaga, Toshiro ♦ Kurz, Samantha ♦ Stephens, Elaine ♦ Baldwin, Timothy C. ♦ Ishii, Tadashi ♦ Napier, Johnathan A. ♦ Weber, Andreas P. M. ♦ Handford, Michael G. ♦ Dupree, Paul
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Heterocyclic Compounds ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Diagnosis ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Metabolism ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Arabidopsis Proteins ♦ Metabolism ♦ Arabidopsis ♦ Glycosphingolipids ♦ Mannose ♦ Membrane Transport Proteins ♦ Salicylic Acid ♦ Genetics ♦ Biological Transport ♦ Cell Wall ♦ Glycosylation ♦ Golgi Apparatus ♦ Guanosine Diphosphate Fucose ♦ Guanosine Diphosphate Mannose ♦ Guanosine Diphosphate Sugars ♦ Immunoblotting ♦ Microscopy, Fluorescence ♦ Mutation ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract The Arabidopsis thaliana protein GOLGI-LOCALIZED NUCLEOTIDE SUGAR TRANSPORTER (GONST1) has been previously identified as a GDP-d-mannose transporter. It has been hypothesized that GONST1 provides precursors for the synthesis of cell wall polysaccharides, such as glucomannan. Here, we show that in vitro GONST1 can transport all four plant GDP-sugars. However, gonst1 mutants have no reduction in glucomannan quantity and show no detectable alterations in other cell wall polysaccharides. By contrast, we show that a class of glycosylated sphingolipids (glycosylinositol phosphoceramides [GIPCs]) contains Man and that this mannosylation is affected in gonst1. GONST1 therefore is a Golgi GDP-sugar transporter that specifically supplies GDP-Man to the Golgi lumen for GIPC synthesis. gonst1 plants have a dwarfed phenotype and a constitutive hypersensitive response with elevated salicylic acid levels. This suggests an unexpected role for GIPC sugar decorations in sphingolipid function and plant defense signaling. Additionally, we discuss these data in the context of substrate channeling within the Golgi.
Description Country affiliation: United kingdom
Author Affiliation: Mortimer JC ( Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-05-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 25
Issue Number 5

Source: WHO-Global Index Medicus