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Author Lee, Yong Woo ♦ Kim, Woo Taek
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Diagnosis ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Dna, Plant ♦ Metabolism ♦ Homologous Recombination ♦ Telomerase ♦ Telomere-binding Proteins ♦ Telomere ♦ Base Sequence ♦ Chemistry ♦ Genetics ♦ Down-regulation ♦ Gene Expression Regulation, Plant ♦ In Situ Hybridization, Fluorescence ♦ Plant Proteins ♦ Protein Binding ♦ Rna Interference ♦ Reverse Transcriptase Polymerase Chain Reaction ♦ Telomere Homeostasis ♦ Tobacco ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract At the 3'-end of telomeres, single-stranded G-overhang telomeric repeats form a stable T-loop. Many studies have focused on the mechanisms that generate and regulate the length of telomere 3' G-strand overhangs, but the roles of G-strand overhang length control in proper T-loop formation and end protection remain unclear. Here, we examined functional relationships between the single-stranded telomere binding protein GTBP1 and G-strand overhang lengths maintained by telomerase in tobacco (Nicotiana tabacum). In tobacco plants, telomerase reverse transcriptase subunit (TERT) repression severely worsened the GTBP1 knockdown phenotypes, which were formally characterized as an outcome of telomere destabilization. TERT downregulation shortened the telomere 3' G-overhangs and increased telomere recombinational aberrations in GTBP1-suppressed plants. Correlatively, GTBP1-mediated inhibition of single-strand invasion into the double-strand telomeric sequences was impaired due to shorter single-stranded telomeres. Moreover, TERT/GTBP1 double knockdown amplified misplaced homologous recombination of G-strand overhangs into intertelomeric regions. Thus, proper G-overhang length maintenance is required to protect telomeres against intertelomeric recombination, which is achieved by the balanced functions of GTBP1 and telomerase activity.
Description Author Affiliation: Lee YW ( Department of Systems Biology, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Korea.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-04-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 25
Issue Number 4

Source: WHO-Global Index Medicus