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Author Ferrario, Silvia ♦ Busscher, Jacqueline ♦ Franken, John ♦ Gerats, Tom ♦ Vandenbussche, Michiel ♦ Angenent, Gerco C. ♦ Immink, Richard G. H.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Flowers ♦ Growth & Development ♦ Genes, Dominant ♦ Genetics ♦ Mads Domain Proteins ♦ Metabolism ♦ Petunia ♦ Plant Leaves ♦ Plant Proteins ♦ Arabidopsis ♦ Physiology ♦ Cloning, Molecular ♦ Gene Deletion ♦ Gene Expression ♦ Gene Expression Profiling ♦ Gene Expression Regulation, Plant ♦ Genes, Plant ♦ Genetic Complementation Test ♦ Phenotype ♦ Phylogeny ♦ Plants, Genetically Modified ♦ Protein Binding ♦ Protoplasts ♦ Time Factors ♦ Two-hybrid System Techniques ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Several genes belonging to the MADS box transcription factor family have been shown to be involved in the transition from vegetative to reproductive growth. The Petunia hybrida MADS box gene UNSHAVEN (UNS) shares sequence similarity with the Arabidopsis thaliana flowering gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1, is expressed in vegetative tissues, and is downregulated upon floral initiation and the formation of floral meristems. To understand the role of UNS in the flowering process, knockout mutants were identified and UNS was expressed ectopically in petunia and Arabidopsis. No phenotype was observed in petunia plants in which UNS was disrupted by transposon insertion, indicating that its function is redundant. Constitutive expression of UNS leads to an acceleration of flowering and to the unshaven floral phenotype, which is characterized by ectopic trichome formation on floral organs and conversion of petals into organs with leaf-like features. The same floral phenotype, accompanied by a delay in flowering, was obtained when a truncated version of UNS, lacking the MADS box domain, was introduced. We demonstrated that the truncated protein is not translocated to the nucleus. Using the overexpression approach with both the full-length and the nonfunctional truncated UNS protein, we could distinguish between phenotypic alterations because of a dominant-negative action of the protein and because of its native function in promoting floral transition.
Description Country affiliation: Netherlands
Author Affiliation: Ferrario S ( Business Unit Bioscience, Plant Research International, 6700 AA, Wageningen, The Netherlands.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2004-06-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 16
Issue Number 6


Source: WHO-Global Index Medicus