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Author Rumeau, Dominique ♦ Bécuwe-Linka, Noëlle ♦ Beyly, Audrey ♦ Louwagie, Mathilde ♦ Garin, Jérôme ♦ Peltier, Gilles
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Macromolecular Substances ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Cell Nucleus ♦ Genetics ♦ Genes, Plant ♦ Nadph Dehydrogenase ♦ Metabolism ♦ Plant Proteins ♦ Plastids ♦ Protein Subunits ♦ Amino Acid Sequence ♦ Arabidopsis ♦ Electron Transport Chain Complex Proteins ♦ Gene Expression Regulation, Plant ♦ Molecular Sequence Data ♦ Mutation ♦ Photosynthesis ♦ Photosystem I Protein Complex ♦ Thylakoids ♦ Tobacco ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract In higher plants, the Ndh complex reduces plastoquinones and is involved in cyclic electron flow around photosystem I, supplying extra-ATP for photosynthesis, particularly under environmental stress conditions. Based on plastid genome sequences, the Ndh complex would contain 11 subunits (NDH-A to -K), but homologies with bacterial complex indicate the probable existence of additional subunits. To identify missing subunits, tobacco (Nicotiana tabacum) NDH-H was His tagged at its N terminus using plastid transformation. A functional Ndh subcomplex was purified by Ni(2+) affinity chromatography and its subunit composition analyzed by mass spectrometry. Five plastid encoded subunits (NDH-A, -H, -I, -J, and -K) were identified as well as three new subunits (NDH-M, -N, and -O) homologous to cyanobacterial and higher plant proteins. Arabidopsis thaliana mutants missing one of these new subunits lack a functional Ndh complex, and NDH-M and NDH-N are not detected in a tobacco transformant lacking the Ndh complex. We discuss the involvement of these three nuclear-encoded subunits in the functional integrity of the plastidial complex.
Description Country affiliation: France
Author Affiliation: Rumeau D ( Département d'Ecophysiologie Végétale et de Microbiologie, Laboratoire d'Ecophysiologie de la Photosynthèse, Centre National de la Recherche Scientifique, Université de la Méditerranée, Saint-Paul-lez-Durance, France.
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2005-01-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 17
Issue Number 1

Source: WHO-Global Index Medicus