Access Restriction

Author Barton, Deborah A. ♦ Vantard, Marylin ♦ Overall, Robyn L.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Discipline Botany ♦ Microtubules ♦ Ultrastructure ♦ Tradescantia ♦ Fluorescent Dyes ♦ Microscopy, Confocal ♦ Microscopy, Electron, Scanning ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Cortical microtubule arrays are highly organized networks involved in directing cellulose microfibril deposition within the cell wall. Their organization results from complex interactions between individual microtubules and microtubule-associated proteins. The precise details of these interactions are often not evident using optical microscopy. Using high-resolution scanning electron microscopy, we analyzed extensive regions of cortical arrays and identified two spatially discrete microtubule subpopulations that exhibited different stabilities. Microtubules that lay adjacent to the plasma membrane were often bundled and more stable than the randomly aligned, discordant microtubules that lay deeper in the cytoplasm. Immunolabeling revealed katanin at microtubule ends, on curves, or at sites along microtubules in line with neighboring microtubule ends. End binding 1 protein also localized along microtubules, at microtubule ends or junctions between microtubules, and on the plasma membrane in direct line with microtubule ends. We show fine bands in vivo that traverse and may encircle microtubules. Comparing confocal and electron microscope images of fluorescently tagged arrays, we demonstrate that optical images are misleading, highlighting the fundamental importance of studying cortical microtubule arrays at high resolution.
Description Country affiliation: Australia
Author Affiliation: Barton DA ( School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-04-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 20
Issue Number 4

Source: WHO-Global Index Medicus