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Author Yamamoto, E. ♦ Zeng, L. ♦ Baird, W. V.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Organic Chemicals ♦ Macromolecular Substances ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Physiological Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Cereals ♦ Genetics ♦ Microtubules ♦ Drug Effects ♦ Tubulin ♦ Alleles ♦ Amino Acid Sequence ♦ Aniline Compounds ♦ Pharmacology ♦ Base Sequence ♦ Dna, Complementary ♦ Chemistry ♦ Dna, Plant ♦ Drug Resistance ♦ Herbicides ♦ Molecular Sequence Data ♦ Mutation ♦ Journal Article ♦ Research Support, Non-u.s. Gov't ♦ Research Support, U.s. Gov't, Non-p.h.s.
Abstract Dinitroaniline herbicides are antimicrotubule drugs that bind to tubulins and inhibit polymerization. As a result of repeated application of dinitroaniline herbicides, highly resistant and intermediately resistant biotypes of goosegrass (Eleusine indica) developed in previously wild-type populations. Three alpha-tubulin cDNA classes (designated TUA1, TUA2, and TUA3) were isolated from each biotype. Nucleotide differences between the susceptible and the resistant (R) alpha-tubulins were identified in TUA1 and TUA2. The most significant differences were missense mutations that occurred in TUA1 of the R and intermediately resistant (I) biotypes. Such mutations convert Thr-239 to Ile in the R biotype and Met-268 to Thr in the I biotype. These amino acid substitutions alter hydrophobicity; therefore, they may alter the dinitroaniline binding property of the protein. These mutations were correlated with the dinitroaniline response phenotypes (Drp). Plants homozygous for susceptibility possessed the wild-type TUA1 allele; plants homozygous for resistance possessed the mutant tua1 allele; and plants heterozygous for susceptibility possessed both wild-type and mutant alleles. Thus, we conclude that TUA1 is at the Drp locus. Using polymerase chain reaction primer-introduced restriction analysis, we demonstrated that goosegrass genomic DNA can be diagnosed for Drp alleles. Although not direct proof, these results suggest that a mutation in an alpha-tubulin gene confers resistance to dinitroanilines in goosegrass.
Description Country affiliation: United States
Author Affiliation: Yamamoto E ( Department of Horticulture, Clemson University, Clemson, South Carolina 29634, USA.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1998-02-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 10
Issue Number 2


Source: WHO-Global Index Medicus