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Author Zuo, J. ♦ Niu, Q. W. ♦ Nishizawa, N. ♦ Wu, Y. ♦ Kost, B. ♦ Chua, N. H.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Eukaryota ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Arabidopsis ♦ Enzymology ♦ Cell Cycle ♦ Genetics ♦ Cellulase ♦ Metabolism ♦ Amino Acid Sequence ♦ Cytology ♦ Base Sequence ♦ Chemistry ♦ Green Fluorescent Proteins ♦ Luminescent Proteins ♦ Molecular Sequence Data ♦ Mutation ♦ Recombinant Fusion Proteins ♦ Sequence Homology, Amino Acid ♦ Journal Article ♦ Research Support, U.s. Gov't, Non-p.h.s.
Abstract The formation of the cell plate, a unique structure in dividing plant cells, is pivotal for cytokinesis. A mutation in the Arabidopsis KORRIGAN (KOR) gene causes the formation of aberrant cell plates, incomplete cell walls, and multinucleated cells, leading to severely abnormal seedling morphology. The mutant, designed kor1-2, was identified as a stronger allele than the previously identified kor1-1, which appears to be defective only in cell elongation. KOR1 encodes an endo-1,4-beta-d-glucanase with a transmembrane domain and two putative polarized targeting signals in the cytosolic tail. When expressed in tobacco BY2 cells, a KOR1-GFP (green fluorescence protein) fusion protein was localized to growing cell plates. Substitution mutations in the polarized targeting motifs of KOR1 caused the fusion proteins to localize to the plasma membrane as well. Expression of these mutant genes in kor1-2 plants complemented only the cell elongation defect but not the cytokinesis defect, indicating that polarized targeting of KOR1 to forming cell plates is essential for cytokinesis. Our results suggest that KOR1 plays a critical role during cytokinesis.
Description Country affiliation: United States
Author Affiliation: Zuo J ( Laboratory of Plant Molecular Biology, The Rockefeller University, New York, New York 10021, USA.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2000-07-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 12
Issue Number 7

Source: WHO-Global Index Medicus