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Author Gavrin, Aleksandr ♦ Kaiser, Brent N. ♦ Geiger, Dietmar ♦ Tyerman, Stephen D. ♦ Wen, Zhengyu ♦ Bisseling, Ton ♦ Fedorova, Elena E.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Macromolecular Substances ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Metabolism ♦ Genetic Phenomena ♦ Microbiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Medicago Truncatula ♦ Cytology ♦ Microbiology ♦ Multiprotein Complexes ♦ Metabolism ♦ Plant Proteins ♦ Symbiosis ♦ Vacuoles ♦ Acids ♦ Biological Markers ♦ Cell Membrane ♦ Gene Expression Regulation, Plant ♦ Hydrogen-ion Concentration ♦ Genetics ♦ Nitrogen Fixation ♦ Plants, Genetically Modified ♦ Protein Transport ♦ Rna Interference ♦ Root Nodules, Plant ♦ Staining And Labeling ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract In legume-rhizobia symbioses, the bacteria in infected cells are enclosed in a plant membrane, forming organelle-like compartments called symbiosomes. Symbiosomes remain as individual units and avoid fusion with lytic vacuoles of host cells. We observed changes in the vacuole volume of infected cells and thus hypothesized that microsymbionts may cause modifications in vacuole formation or function. To examine this, we quantified the volumes and surface areas of plant cells, vacuoles, and symbiosomes in root nodules of Medicago truncatula and analyzed the expression and localization of VPS11 and VPS39, members of the HOPS vacuole-tethering complex. During the maturation of symbiosomes to become N2-fixing organelles, a developmental switch occurs and changes in vacuole features are induced. For example, we found that expression of VPS11 and VPS39 in infected cells is suppressed and host cell vacuoles contract, permitting the expansion of symbiosomes. Trafficking of tonoplast-targeted proteins in infected symbiotic cells is also altered, as shown by retargeting of the aquaporin TIP1g from the tonoplast membrane to the symbiosome membrane. This retargeting appears to be essential for the maturation of symbiosomes. We propose that these alterations in the function of the vacuole are key events in the adaptation of the plant cell to host intracellular symbiotic bacteria.
Description Country affiliation: Netherlands
Author Affiliation: Gavrin A ( Laboratory of Molecular Biology, Wageningen University, 6708PB Wageningen, The Netherlands.); Kaiser BN ( School of Agriculture, Food, and Wine, Waite Research Institute, University of Adelaide, Adelaide, South Australia 5064, Australia.); Geiger D ( Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, D-97082 Würzburg, Germany.); Tyerman SD ( School of Agriculture, Food, and Wine, Waite Research Institute, University of Adelaide, Adelaide, South Australia 5064, Australia.); Wen Z ( School of Agriculture, Food, and Wine, Waite Research Institute, University of Adelaide, Adelaide, South Australia 5064, Australia.); Bisseling T ( Laboratory of Molecular Biology, Wageningen University, 6708PB Wageningen, The Netherlands College of Science, King Saud University, Riyadh 11451, Saudi Arabia.); Fedorova EE ( Laboratory of Molecular Biology, Wageningen University, 6708PB Wageningen, The Netherlands elena.fedorova@wur.nl.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-09-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 26
Issue Number 9


Source: WHO-Global Index Medicus