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Author Milioni, Dimitra ♦ Sado, Pierre-Etienne ♦ Stacey, Nicola J. ♦ Roberts, Keith ♦ McCann, Maureen C.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Heterocyclic Compounds ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Metabolism ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Asteraceae ♦ Genetics ♦ Cell Differentiation ♦ Transcription, Genetic ♦ Physiology ♦ Biological Transport ♦ Drug Effects ♦ Cytokinins ♦ Pharmacology ♦ Dna, Complementary ♦ Chemistry ♦ Expressed Sequence Tags ♦ Gene Expression Regulation, Plant ♦ Indoleacetic Acids ♦ Molecular Sequence Data ♦ Plant Growth Regulators ♦ Plant Leaves ♦ Cytology ♦ Plant Proteins ♦ Metabolism ♦ Polymorphism, Restriction Fragment Length ♦ Reverse Transcriptase Polymerase Chain Reaction ♦ Sequence Analysis, Dna ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Isolated mesophyll cells from Zinnia elegans are induced by auxin and cytokinin to form tracheary elements (TEs) in vitro with high synchrony. To reveal the changing patterns of gene expression during the 48 h of transdifferentiation from mesophyll to TE cell fate, we used a cDNA-amplified fragment length polymorphism approach to generate expression profiles of >30,000 cDNA fragments. Transcriptional changes of 652 cDNA fragments were observed, of which 304 have no previously described function or sequence identity. Sixty-eight genes were upregulated within 30 min of induction and represent key candidates for the processes that underlie the early stages of commitment and differentiation to a TE cell fate.
Description Country affiliation: United kingdom
Author Affiliation: Milioni D ( Department of Cell and Developmental Biology, John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2002-11-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 14
Issue Number 11


Source: WHO-Global Index Medicus