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Author Overvoorde, P. J. ♦ Frommer, W. B. ♦ Grimes, H. D.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Chemical Phenomena ♦ Metabolism ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Carrier Proteins ♦ Metabolism ♦ Membrane Transport Proteins ♦ Plant Lectins ♦ Plant Proteins ♦ Saccharomyces Cerevisiae ♦ Soybean Proteins ♦ Soybeans ♦ Sucrose ♦ 2,4-dinitrophenol ♦ Base Sequence ♦ Biological Transport ♦ Carbon Radioisotopes ♦ Carbonyl Cyanide M-chlorophenyl Hydrazone ♦ Pharmacology ♦ Biosynthesis ♦ Cell Membrane ♦ Cloning, Molecular ♦ Dna, Complementary ♦ Dinitrophenols ♦ Genotype ♦ Kinetics ♦ Molecular Sequence Data ♦ Oligodeoxyribonucleotides ♦ Plasmids ♦ Recombinant Proteins ♦ Restriction Mapping ♦ Genetics ♦ Substrate Specificity ♦ Sulfhydryl Reagents ♦ Comparative Study ♦ Journal Article ♦ Research Support, U.s. Gov't, Non-p.h.s.
Abstract Heterologous expression of a cDNA encoding a 62-kD soybean sucrose binding protein in yeast demonstrates that this protein, independent of other plant proteins, mediates sucrose uptake across the plasma membrane. Sucrose binding protein-mediated sucrose uptake is nonsaturable up to 30 mM sucrose, is specific for sucrose, and is relatively insensitive to treatment with sulfhydryl-modifying reagents. Alteration of the external pH or pretreatment of the yeast cells with protonophores did not significantly affect the rate of 14C-sucrose uptake. This demonstrates that sucrose binding protein-mediated sucrose uptake is not dependent on H+ movement and delineates it from other plant sucrose transporters. Physiological characterization of sucrose uptake into higher plant cells has shown the presence of both saturable and nonsaturable uptake components. The nonsaturable mechanism is relatively insensitive to external pH, pretreatment with protonophores, and treatment with sulfhydryl-modifying reagents. Sucrose binding protein-mediated sucrose uptake in yeast mimics this physiologically described, but mechanistically undefined, nonsaturable sucrose uptake mechanism in higher plants. Functional characterization of the sucrose binding protein thus defines both a novel component of sucrose uptake and provides important insight into this nonsaturable sucrose uptake mechanism, which has remained enigmatic since its physiological description.
Description Country affiliation: United States
Author Affiliation: Overvoorde PJ ( Department of Botany, Washington State University, Pullman 99164-4238, USA.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1996-02-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 8
Issue Number 2

Source: WHO-Global Index Medicus