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Author Poulsen, Lisbeth Rosager ♦ López-Marqués, Rosa Laura ♦ McDowell, Stephen C. ♦ Okkeri, Juha ♦ Licht, Dirk ♦ Schulz, Alexander ♦ Pomorski, Thomas ♦ Harper, Jeffrey F. ♦ Palmgren, Michael Gjedde
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Enzymes and Coenzymes ♦ Lipids ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Metabolism ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Adenosine Triphosphatases ♦ Metabolism ♦ Arabidopsis Proteins ♦ Arabidopsis ♦ Golgi Apparatus ♦ Secretory Vesicles ♦ Genetics ♦ Amino Acid Sequence ♦ Ultrastructure ♦ Biological Transport ♦ Molecular Sequence Data ♦ Phospholipids ♦ Plant Roots ♦ Plant Shoots ♦ Plants, Genetically Modified ♦ Sequence Homology, Amino Acid ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Vesicle budding in eukaryotes depends on the activity of lipid translocases (P(4)-ATPases) that have been implicated in generating lipid asymmetry between the two leaflets of the membrane and in inducing membrane curvature. We show that Aminophospholipid ATPase3 (ALA3), a member of the P(4)-ATPase subfamily in Arabidopsis thaliana, localizes to the Golgi apparatus and that mutations of ALA3 result in impaired growth of roots and shoots. The growth defect is accompanied by failure of the root cap to release border cells involved in the secretion of molecules required for efficient root interaction with the environment, and ala3 mutants are devoid of the characteristic trans-Golgi proliferation of slime vesicles containing polysaccharides and enzymes for secretion. In yeast complementation experiments, ALA3 function requires interaction with members of a novel family of plant membrane-bound proteins, ALIS1 to ALIS5 (for ALA-Interacting Subunit), and in this host ALA3 and ALIS1 show strong affinity for each other. In planta, ALIS1, like ALA3, localizes to Golgi-like structures and is expressed in root peripheral columella cells. We propose that the ALIS1 protein is a beta-subunit of ALA3 and that this protein complex forms an important part of the Golgi machinery required for secretory processes during plant development.
Description Country affiliation: Denmark
Author Affiliation: Poulsen LR ( Danish National Research Foundation, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-03-01
Publisher Place United States
e-ISSN 1531298X
Volume Number 20
Issue Number 3

Source: WHO-Global Index Medicus