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Author Kobayashi, Tamaki ♦ Takahara, Manabu ♦ Miyagishima, Shin-ya ♦ Kuroiwa, Haruko ♦ Sasaki, Narie ♦ Ohta, Niji ♦ Matsuzaki, Motomichi ♦ Kuroiwa, Tsuneyoshi
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Bacteria ♦ Organisms ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Algal Proteins ♦ Metabolism ♦ Chloroplasts ♦ Dna, Chloroplast ♦ Genetics ♦ Rhodophyta ♦ Saccharomyces Cerevisiae Proteins ♦ Amino Acid Sequence ♦ Bacterial Proteins ♦ Dna-binding Proteins ♦ Escherichia Coli ♦ Genetic Complementation Test ♦ Immunoblotting ♦ Molecular Sequence Data ♦ Mutation ♦ Sequence Homology, Amino Acid ♦ Transcription Factors ♦ Comparative Study ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Chloroplast DNA (cpDNA) is packed into discrete structures called chloroplast nucleoids (cp-nucleoids). The structure of cpDNA is thought to be important for its maintenance and regulation. In bacteria and mitochondria, histone-like proteins (such as HU and Abf2, respectively) are abundant and play important roles in DNA organization. However, a primary structural protein has yet to be found in cp-nucleoids. Here, we identified an abundant DNA binding protein from isolated cp-nucleoids of the primitive red alga Cyanidioschyzon merolae. The purified protein had sequence homology with the bacterial histone-like protein HU, and it complemented HU-lacking Escherichia coli mutants. The protein, called HC (histone-like protein of chloroplast), was encoded by a single gene (CmhupA) in the C. merolae chloroplast genome. Using immunofluorescence and immunoelectron microscopy, we demonstrated that HC was distributed uniformly throughout the entire cp-nucleoid. The protein was expressed constitutively throughout the cell and the chloroplast division cycle, and it was able to condense DNA. These results indicate that HC, a bacteria-derived histone-like protein, primarily organizes cpDNA into the nucleoid.
Description Country affiliation: Japan
Author Affiliation: Kobayashi T ( Department of Biological Science, Graduate School of Science, University of Tokyo, Tokyo 113-0033, Japan. tamaki@biol.s.u-tokyo.ac.jp)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2002-07-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 14
Issue Number 7


Source: WHO-Global Index Medicus