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Author Zhang, Qun ♦ Lin, Feng ♦ Mao, Tonglin ♦ Nie, Jianing ♦ Yan, Min ♦ Yuan, Ming ♦ Zhang, Wenhua
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Lipids ♦ Amino Acids, Peptides, and Proteins ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Physiological Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Arabidopsis Proteins ♦ Metabolism ♦ Arabidopsis ♦ Genetics ♦ Gene Expression Regulation, Plant ♦ Microtubules ♦ Phosphatidic Acids ♦ Sodium Chloride ♦ Pharmacology ♦ Amino Acid Sequence ♦ Drug Effects ♦ Physiology ♦ Ultrastructure ♦ Base Sequence ♦ Cell Membrane ♦ Dinitrobenzenes ♦ Microtubule-associated Proteins ♦ Models, Molecular ♦ Molecular Sequence Data ♦ Mutation ♦ Phospholipase D ♦ Protein Binding ♦ Salt-tolerance ♦ Seedling ♦ Sequence Alignment ♦ Sequence Analysis, Dna ♦ Signal Transduction ♦ Stress, Physiological ♦ Sulfanilamides ♦ Tubulin Modulators ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Membrane lipids play fundamental structural and regulatory roles in cell metabolism and signaling. Here, we report that phosphatidic acid (PA), a product of phospholipase D (PLD), regulates MAP65-1, a microtubule-associated protein, in response to salt stress. Knockout of the PLD 1 gene resulted in greater NaCl-induced disorganization of microtubules, which could not be recovered during or after removal of the stress. Salt affected the association of MAP65-1 with microtubules, leading to microtubule disorganization in pld 1cells, which was alleviated by exogenous PA. PA bound to MAP65-1, increasing its activity in enhancing microtubule polymerization and bundling. Overexpression of MAP65-1 improved salt tolerance of Arabidopsis thaliana cells. Mutations of eight amino acids in MAP65-1 led to the loss of its binding to PA, microtubule-bundling activity, and promotion of salt tolerance. The pld 1 map65-1 double mutant showed greater sensitivity to salt stress than did either single mutant. These results suggest that PLD 1-derived PA binds to MAP65-1, thus mediating microtubule stabilization and salt tolerance. The identification of MAP65-1 as a target of PA reveals a functional connection between membrane lipids and the cytoskeleton in environmental stress signaling.
Description Country affiliation: China
Author Affiliation: Zhang Q ( College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-11-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 24
Issue Number 11


Source: WHO-Global Index Medicus