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Author Steffens, Bianka ♦ Sauter, Margret
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Computer science, information & general works ♦ Library & information sciences ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Plant Structures ♦ Anatomy ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Information Science ♦ Information Science
Subject Keyword Discipline Botany ♦ Cell Death ♦ Ethylenes ♦ Metabolism ♦ Hydrogen Peroxide ♦ Oryza Sativa ♦ Plant Epidermis ♦ Cytology ♦ Cyclopropanes ♦ Pharmacology ♦ Gene Expression Profiling ♦ Gene Expression Regulation, Plant ♦ Molecular Sequence Data ♦ Oligonucleotide Array Sequence Analysis ♦ Genetics ♦ Growth & Development ♦ Plant Roots ♦ Rna, Plant ♦ Signal Transduction ♦ Journal Article
Abstract Rice (Oryza sativa) forms adventitious root primordia at stem nodes during normal development. Root emergence is preceded by ethylene-induced, H(2)O(2)-mediated local death of epidermal cells. Exogenous H(2)O(2) or enhancement of endogenous H(2)O(2) promoted epidermal cell death in a dose-dependent manner. Inhibition of NADPH oxidase lowered ethylene-induced cell death rates. Inhibition of ethylene perception by 1-methylcyclopropene did not abolish H(2)O(2)-induced cell death, indicating that H(2)O(2) acts downstream of ethylene. Microarray studies of epidermal cells that undergo cell death identified 61 genes coregulated by the ethylene-releasing compound ethephon and by H(2)O(2), supporting a joint signaling pathway. Regulation of the ethylene biosynthetic genes 1-Aminocyclopropane-1-Carboxylate Oxidase1 and Ethylene Overproducer-Like1 and downregulation of Metallothionein2b (MT2b), which encodes a reactive oxygen scavenger, indicated mutual enhancement of ethylene and H(2)O(2) signaling. Analysis of MT2b knockdown mutants showed that cell death rates were inversely related to MT2b transcript abundance. Epidermal cells above adventitious roots have a morphological and molecular identity distinct from other epidermal cells. Pro-death signals regulated several transcription factor genes with a proposed function in cell type specification. It is hypothesized that induction of cell death is dependent on epidermal cell identity.
Description Country affiliation: Germany
Author Affiliation: Steffens B ( Botanisches Institut, Universität Kiel, 24118 Kiel, Germany.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-01-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 21
Issue Number 1


Source: WHO-Global Index Medicus