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Author Lei, Lei ♦ Li, Shundai ♦ Du, Juan ♦ Bashline, Logan ♦ Gu, Ying
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher American Society of Plant Biologists
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Macromolecular Substances ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Cell Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Arabidopsis Proteins ♦ Physiology ♦ Arabidopsis ♦ Metabolism ♦ Cellulose ♦ Biosynthesis ♦ Microtubule-associated Proteins ♦ Microtubules ♦ Cytology ♦ Genetics ♦ Analysis ♦ Carrier Proteins ♦ Cell Enlargement ♦ Ultrastructure ♦ Journal Article ♦ Research Support, U.s. Gov't, Non-p.h.s.
Abstract Anisotropic plant cell growth depends on the coordination between the orientation of cortical microtubules and the orientation of nascent cellulose microfibrils. Cellulose synthase interactive1 (CSI1) is a key scaffold protein that guides primary cellulose synthase complexes (CSCs) along cortical microtubules during cellulose biosynthesis. Here, we investigated the function of the CSI1-like protein, CSI3, in Arabidopsis thaliana. Similar to CSI1, CSI3 associates with primary CSCs in vitro, colocalizes with CSCs in vivo, and exhibits the same plasma membrane localization and bidirectional motility as CSI1. However, ProCSI1:GFP-CSI3 cannot complement the anisotropic cell growth defect in csi1 mutants, suggesting that CSI3 is not functionally equivalent to CSI1. Also, the colocalization ratio between CSI1 and CSI3 is low, which may suggest heterogeneity within the CSC population. csi1 csi3 double mutants showed an enhanced cell expansion defect as well as an additive reduction of CSC velocities, and CSI3 dynamics are dependent on CSI1 function. We propose that CSI3 is an important regulator of plant cellulose biosynthesis and plant anisotropic cell growth that modulates the velocity of CSCs in both a microtubule-dependent and microtubule-independent manner.
Description Author Affiliation: Lei L ( Department of Biochemistry and Molecular Biology, Pensylvania State University, University Park, Pensylvania 16802.)
ISSN 10404651
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-12-01
Publisher Place United States
e-ISSN 1531298X
Journal THE PLANT CELL ONLINE
Volume Number 25
Issue Number 12


Source: WHO-Global Index Medicus